By group 73


Proper Microscope Use

If you're going to study life science, you will most likely use a microscope, and it is vital that you know how to use one properly in class. When you are going to get a microscope, always carry it with two hands. One hand should be under the base and the other should be holding the arm of the microscope. When you put it down, make sure it is at least ten centimeters away from each edge of the table. In other words, don't keep it close to the edge. This will prevent hurting your foot or breaking the microscope. It is also importiant to make sure that the microscope's arm is facing towards you so you or the arm doesn't block the light. Take the cover off carefully so you don't hit anything, and direct the mirror at the nearest light, so it goes through the diaphragm so you can see the object on the slide. Make sure that you have a bright light coming through the eyepiece, and that nothing is blocking your light. If you don't have a mirror then you probably have a electric lamp that you must turn on the light by plugging the plug in. Now you can select the slide you wish to view, now place it on the stage. Then, clip it on with the stage clips to the left and right of the slide, making sure that the slide is centered. Now you can begin to focus the object on low power with the coarse adjustment knob. Next, use the fine adjustment knob, if you have one, for the final adjustments. After that, do the same thing on medium and high power. you should never start with the microscope on high power; always start on low power. While on high power, your object will be very large, but be careful while focusing it because you could crack the glass of the slide and break it. That is because the high objective lens is longer than the rest. Also you will see less of your object if you use a higher power, so if you do not see your object either move the slide around a bit until you find it or go back to a lower power. You will also notice that your slide looks upside down when you look at it through the eyepiece, and when you are trying to go to the left of the slide you have to push it to the right in order for it to go left. The first tip would be to not look through the microscope while your adjusting the knobs on high power because you need to watch the slide to make sure it doesn't crack. Then, observe your object and record information about it. When it's time to clean up, remove the slide, turn the lens on low power and adjust the stage so that it is all the way down. Finally put the cover back on, and bring it back to it's proper storing place, and call it a day. Once you know all these procedures, then you will be able to properly use a microscope in class without any problems.

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